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Epcam Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - Epcam Antibody (C-term) AJ1263a
    A. Western blot analysis on A431 cell lysate using anti-Epithelial Antigen (Ep-CAM) (C-term) RabMAb (Cat. #AJ1263a); dilution 1:2,500.
  • IHC - Epcam Antibody (C-term) AJ1263a
    B. Immunohistochemical analysis of paraffin-embedded stomach carcinoma using anti-Epithelial Antigen (Ep-CAM) (C-term) RabMAb (Cat. #AJ1263a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16422
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E144
Calculated MW 34932 Da
Gene ID 4072
Other Names Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Target/Specificity A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Ep-CAM was used as immunogen.
Dilution WB~~1:500~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEpcam Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms GA733-2, M1S2, M4S1, MIC18, TACSTD1, TRO
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Cellular Location Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction
Tissue Location Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Research Areas
Citations (0)

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Epithelial cell adhesion molecule (Ep-CAM) is a monomeric membrane glycoprotein that is expressed on virtually all epithelial cell membranes and on a variety of epithelial carcinomas (1-3). Ep-CAM is overexpressed in a variety of human carcinomas and is, therefore, a potential therapeutic target for human solid tumors (4). Ep-CAM contains an extracellular domain with two epidermal growth factor-like repeats, followed by a cysteine-poor region, which are necessary for the adhesion properties of the molecule (5).


1. Tomita, Y., et al. Molecular identification of a human carcinoma-associated glycoprotein antigen recognized by mouse monoclonal antibody FU-MK-1. J. Cancer Res. 91: 231
2. Trebak, M., et al. Oligomeric state of the colon carcinoma-associated glycoprotein GA733-2 (Ep-CAM/EGP40) and its role in GA733-mediated homotypic cell-cell adhesion. J. Biol. Chem. 276: 2299
3. Simon, B., et al. Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc Natl Acad Sci U S A. 87: 2755
4. Szala, S., et al. Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2. Proc Natl Acad Sci U S A. 87: 3542
5. Balzar, M., et al. Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions. Mol. Cell. Biol. 21: 2570

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Cat# AJ1263a
(40 western blots)
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