|Calculated MW||55065 Da|
|Other Names||Erythropoietin receptor, EPO-R, EPOR|
|Target/Specificity||A phospho specific peptide corresponding to residues surrounding Tyrosine 485 of human EpoR was used as an immunogen. The antibody only detects EpoR phosphorylated at Tyrosine 485.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EpoR Antibody Phospho (pY485) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early- stage erythroid progenitor cells|
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Provided below are standard protocols that you may find useful for product applications.
Erythropoietin (Epo) is a hematopoietic cytokine that is a potent regulator of the viability, differentiation and proliferation of erythroid progenitor cells (1, 3). Epo acts on its target cells by inducing homodimerization of the erythropoietin receptor (EpoR), thereby triggering intracellular signaling cascades. The EpoR encompasses eight tyrosine motifs on its cytoplasmic tail that have been shown to recruit a number of regulatory proteins (1). EpoR shares conserved features in its extracellular and cytoplasmic domains with other family members of cytokine and growth factor receptors (2). Alterations of the Epo/EpoR system have recently been shown to be involved in the pathogenesis of familial erythrocytosis and polycythaemia vera (PV) (3).
1. H鰎tner M, et al. Eur J Biochem. 269(10):2516-26, 2002
2. Middleton SA, et al. J Biol Chem. 271(24):14045-54, 1996
3. Hess G, et al. Br J Haematol. 88(4):794-802, 1994
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