|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||60293 Da|
|Other Names||Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1|
|Target/Specificity||A synthetic peptide corresponding to residues in human Epsin-1 was used as an immunogen|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Epsin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Membrane, clathrin-coated pit Note=Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity)|
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Provided below are standard protocols that you may find useful for product applications.
Epsin-1 is a cytosolic adaptor protein and interacting partner for the EH domain-containing region of Eps15. It is involved in the regulation of clathrin-mediated endocytosis via its direct interactions with clathrin, the clathrin adaptor AP-2, and Eps15 (1-3). Epsin-1 is a cargo-specific adaptor for influenza virus entry through the clathrin-mediated pathway. In addition to being a clathrin-interacting protiein, Epsin-1 is also an ubiquitin-, and phospholipid-interacting protein (4). It also may function in a signaling pathway connecting the endocytic machinery to the regulation of nuclear function (1).
1. Hyman J, et al. J Cell Biol. 149(3):537-46, 2000
2. Timsit YE, et al. Biochem Biophys Res Commun. 328(2):550-9, 2005
3. Rosenthal JA, et al. J Biol Chem. 274(48):33959-65, 1999
4. Chen C, et al. PNAS 105(33):11790-11795, 2008
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