Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Epsin-1 Antibody   

Epsin-1 Antibody

Rabbit Monoclonal Antibody

     
  • WB - Epsin-1 Antibody AJ1267a
    A. Western blot analysis on (A) SH-SY5Y and (B) HeLa cell lysates using anti-Epsin-1 RabMAb (Cat. #AJ1267a), dilution 1:2000.
  • IF - Epsin-1 Antibody AJ1267a
    B. Immunofluorescent staining of SH-SY5Y cells using anti-Epsin1 RabMAb(Cat. #AJ1267a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q9Y6I3
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3023
Calculated MW 60293 Da
Gene ID 29924
Other Names Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1
Target/Specificity A synthetic peptide corresponding to residues in human Epsin-1 was used as an immunogen
Dilution WB~~1:5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEpsin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPN1
Function Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Membrane, clathrin-coated pit Note=Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity)
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.

Background

Epsin-1 is a cytosolic adaptor protein and interacting partner for the EH domain-containing region of Eps15. It is involved in the regulation of clathrin-mediated endocytosis via its direct interactions with clathrin, the clathrin adaptor AP-2, and Eps15 (1-3). Epsin-1 is a cargo-specific adaptor for influenza virus entry through the clathrin-mediated pathway. In addition to being a clathrin-interacting protiein, Epsin-1 is also an ubiquitin-, and phospholipid-interacting protein (4). It also may function in a signaling pathway connecting the endocytic machinery to the regulation of nuclear function (1).

References

1. Hyman J, et al. J Cell Biol. 149(3):537-46, 2000
2. Timsit YE, et al. Biochem Biophys Res Commun. 328(2):550-9, 2005
3. Rosenthal JA, et al. J Biol Chem. 274(48):33959-65, 1999
4. Chen C, et al. PNAS 105(33):11790-11795, 2008

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Discontinued
Cat# AJ1267a
Size:
(40 western blots)
Alternative Products:

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions