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ER alpha Antibody Phospho (pS118)

Rabbit Monoclonal Antibody

     
  • WB - ER alpha Antibody Phospho (pS118) AJ1268d
    A. Western blot analysis on MCF7 cell lysate using anti-Phospho-ER alpha (S118) RabMAb (Cat. #AJ1268d); dilution 1:1,000. Cell lysates were (1) untreated, or (2) treated with b-Estradiol and EGF.
  • IHC - ER alpha Antibody Phospho (pS118) AJ1268d
    B. Immunohistochemical staining of untreated paraffin-embedded human breast adenocarcinoma using anti-Phospho-ER alpha (S118) RabMAb (Cat. #AJ1268d).
  • IHC - ER alpha Antibody Phospho (pS118) AJ1268d
    C. Immunohistochemical staining of Phosphatase-treated paraffin-embedded human breast adenocarcinoma using anti-Phospho-ER alpha (S118) RabMAb (Cat. #AJ1268d).
  • IF - ER alpha Antibody Phospho (pS118) AJ1268d
    D. Immunofluorescent staining of untreated MCF-7 cells using anti-Phospho-ER alpha (S118) RabMAb (Cat. #AJ1268d).
  • IF - ER alpha Antibody Phospho (pS118) AJ1268d
    E. Immunofluorescent staining of Phosphatase-treated MCF-7 cells using anti-Phospho-ER alpha (S118) RabMAb (Cat. #AJ1268d).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P03372
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names E91
Calculated MW 66216 Da
Gene ID 2099
Other Names Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser118 of human ERalpha was used as immunogen. The antibody only detects ERalpha phosphorylated on Serine 118.
Dilution WB~~1:500
IHC~~1:50
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsER alpha Antibody Phospho (pS118) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ESR1
Synonyms ESR, NR3A1
Function Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
Cellular Location Isoform 1: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:12682286}. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated
Tissue Location Widely expressed. Isoform 3 is not expressed in the pituitary gland.
Research Areas
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Background

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

References

1. Pakdel, F., et al. Identification of charged residues in an N-terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol. Endocrinol. 7: 1408
2. Sheeler, C.Q., et al. Mutation of serines 104, 106, and 118 inhibits dimerization of the human estrogen receptor in yeast. Endocr. Res. 29: 237
3. Kato, S., et al. Activation of the estrogen receptor through phosphorylation by mitogen-activated protein kinase. Science 270: 1491

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Discontinued
Cat# AJ1268d
Size:
(40 western blots)
Alternative Products:

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