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ER beta AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1269a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
ER beta Antibody - Product info | |
| Application | WB, IF
|
| Primary Accession | Q92731 |
| Reactivity | Human |
| Clone Names | EPR3777 |
| Calculated MW | 59216 Da |
| Gene ID 2100 | |
| Target/Specificity A synthetic peptide corresponding to N-terminus of human ER beta was used as an immunogen. | |
| Dilution WB~~1:50000~200000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ER beta Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ER beta Antibody - Protein Information | |
| Name ESR2 | |
| Synonyms ESTRB, NR3A2 | |
| Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA- binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual | |
| Cellular Location Nucleus. | |
| Tissue Location Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain | |
ER beta Antibody - Related products
AP1702c: ESR2 Antibody (Center)
ER beta Antibody - Application data
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Fig 1. Western blot analysis on (A) MCF-7, (B) HeLa cell lysates using anti-ER beta RabMAb (Cat. #AJ1269a).
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Fig 2. Immunofluorescent staining of HeLa cells using anti-ER beta RabMAb (Cat. #AJ1269a)
ER beta Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Estrogen Receptor beta (ER beta) is a nuclear hormone receptor which is highly homologous to ER alpha. It functions as a ligand-activated transcription factor, binding to estrogens and activation expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (1). Studies have shown ER beta play a role in modulation of the effects of ER alpha, possibly by inhibiting the transcription activation of ER alpha (2). ER beta is expressed in many tissues, including human thymus, spleen, ovary and testis. It is also highly expressed in endothelial and vascular smooth muscles cells, and the expression of ER beta in these areas has a positive correlation with increased coronary artery plaque (3).
REFERENCES
1. The UniProt Consortium. The Universal Protein Resource (UniProt) Nucleic Acids Res. 37:D169-D174(2009). 2. De Voogd et al., Arch Ophthalmo, 126(1):110 ? 114, 2008. 3. Chen YC et al., Cancer Epidemiol Biomarkers Prev. 16(10):1973-1981, 2007.
