Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ER beta Antibody   

ER beta Antibody

Rabbit Monoclonal Antibody

  • WB - ER beta Antibody AJ1269a
    Fig 1. Western blot analysis on (A) MCF-7, (B) HeLa cell lysates using anti-ER beta RabMAb (Cat. #AJ1269a).
  • IF - ER beta Antibody AJ1269a
    Fig 2. Immunofluorescent staining of HeLa cells using anti-ER beta RabMAb (Cat. #AJ1269a)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92731
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3777
Calculated MW 59216 Da
Gene ID 2100
Other Names Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2
Target/Specificity A synthetic peptide corresponding to N-terminus of human ER beta was used as an immunogen.
Dilution WB~~1:50000~200000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsER beta Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ESR2
Synonyms ESTRB, NR3A2
Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA- binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:19126643}
Tissue Location Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Estrogen Receptor beta (ER beta) is a nuclear hormone receptor which is highly homologous to ER alpha. It functions as a ligand-activated transcription factor, binding to estrogens and activation expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (1). Studies have shown ER beta play a role in modulation of the effects of ER alpha, possibly by inhibiting the transcription activation of ER alpha (2). ER beta is expressed in many tissues, including human thymus, spleen, ovary and testis. It is also highly expressed in endothelial and vascular smooth muscles cells, and the expression of ER beta in these areas has a positive correlation with increased coronary artery plaque (3).


1. The UniProt Consortium. The Universal Protein Resource (UniProt) Nucleic Acids Res. 37:D169-D174(2009). 2. De Voogd et al., Arch Ophthalmo, 126(1):110 ? 114, 2008. 3. Chen YC et al., Cancer Epidemiol Biomarkers Prev. 16(10):1973-1981, 2007.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# AJ1269a
(40 western blots)
Alternative Products:

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions