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Phospho-MAPK3-pT202/T204 AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1271b | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Phospho-MAPK3-pT202/T204 Antibody - Product info | |
| Application | WB
|
| Primary Accession | p27361 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP197Y |
| Calculated MW | 43136 Da |
| Gene ID 5595 | |
| Other Names MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, p44-ERK1;ERT2;p44-MAPK;Microtubule-associated protein 2 kinase | |
| Target/Specificity A phospho specific peptide corresponding to residues surrounding Threonine 202 and 204 of human Erk1/p44. This antibody detects Erk1/p44 phosphorylated at Threonine 202 and 204. | |
| Dilution WB~~1:1000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-MAPK3-pT202/T204 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-MAPK3-pT202/T204 Antibody - Protein Information | |
| Name MAPK3 | |
| Synonyms ERK1, PRKM3 | |
| Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10) Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade | |
| Cellular Location Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization | |
Phospho-MAPK3-pT202/T204 Antibody - Related products
AP3605a: Phospho-MAPK3-pT202 Antibody
AP3606a: Phospho-MAPK3-pY204 Antibody
AP3607a: Bi-phospho-ERK1/2-T202/Y204 Antibody
AP7251a: MAPK3 Antibody (N-term)
AP7251b: MAPK3 Antibody (C-term)
AP7251d: MAPK3 Antibody (T202)
AP7251e: MAPK3 Antibody (Y204)
AP7251f: MAPK3 Antibody (D192)
AP7500a: MAPK3 Antibody (N-term)
RI13190: MAPK3 predesign siRNA
LY10587a: MAPK3 Over-expression Lysate
BP3605a: Phospho-ERK1-pT202 Antibody Blocking Peptide
BP3606a: Phospho-ERK1-pY204 Antibody Blocking Peptide
BP3607a: Phospho-ERK1-pT202/pY204 Antibody Blocking Peptide
BP7251a: MAPK3 Antibody (N-term) Blocking Peptide
BP7251b: MAPK3 Antibody (C-term) Blocking Peptide
BP7251d: MAPK3 Antibody (T202) Blocking Peptide
BP7251e: MAPK3 Antibody (Y204) Blocking Peptide
BP7251f: ERK1 Antibody (D192) Blocking Peptide
BP7500a: ERK1 Antibody (N-term) Blocking Peptide
AT2789a: MAPK3 Antibody (monoclonal) (M01)
AT2790a: MAPK3 Antibody (monoclonal) (M02)
AT2791a: MAPK3 Antibody (monoclonal) (M03)
AJ1271a: MAPK3 Antibody (C-term)
AJ1271b: Phospho-MAPK3-pT202/T204 Antibody
AJ1458a: MAPK3 Antibody (N-term)
AJ1602a: Phospho-Erk1 (pT202/pY204) / Erk2 (pT185/pY187) Antibody
Phospho-MAPK3-pT202/T204 Antibody - Application data
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A. Western blot analysis on A431cell lysates using anti-Phospho-Erk1/p44 (pT202/204) RabMAb (Cat. #AJ1271b), 1:2000 dilution. Cells were either (A) untreated (B) treated with EGF.
Phospho-MAPK3-pT202/T204 Antibody - Research Areas
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BACKGROUND
Extra cellular signal-regulated kinases Erk1 (p44 MAPK) Serine/Threonine protein kinase is part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction (1,2,3). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) . Erk1 is also known to autophosphorylate on tyrosine (4).
REFERENCES
1. Charest, D.L, et al. Mol Cell Biol. 13: 4679
2. Marshall, C.J. Cell 80: 179
3. Brott, B. K. et al. Cell Growth Differ. 4, 921-9294, 1993
4. Payne, D.M., et al. EMBO J. 10: 885
