|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||82681 Da|
|Other Names||Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, Extracellular signal-regulated kinase 3, ERK-3, MAP kinase isoform p97, p97-MAPK, MAPK6, ERK3, PRKM6|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminal of human ERK3 protein was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Erk3/MAPK6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following interaction with MAPKAPK5.|
|Tissue Location||Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts|
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Provided below are standard protocols that you may find useful for product applications.
ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4).
1. Pearson G et al. Endocr. Rev. 22:153-183, 2001.
2. Bind, E. et al. Mol. Biol. Cell 15:4457-4466, 2004.
3. Cheng M et al. J Biol Chem. 12:271:8951-8958, 1996.
4. Boulton TG et al. Cell 65:663-675, 1991.
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