|Calculated MW||59232 Da|
|Other Names||Eyes absent homolog 2, EYA2, EAB1|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus region of human EYA2 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EYA2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr- 142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1.|
|Cellular Location||Cytoplasm. Nucleus. Note=Retained in the cytoplasm via interaction with GNAZ and GNAI2. Interaction with SIX1, SIX2, SIX4 or SIX5 is required for translocation to the nucleus|
|Tissue Location||Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart|
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EYA2 (eyes absent homolog 2) is a human homologue of the Drosophila Eya transcription co-activator. EYA2 is a cytosolic tyrosine phosphatase which translocates to the nucleaus by the Six homeodomain-containing family of proteins, where it becomes cofactor for the transcription factor SIX1. Specifically, EYA2 dephosphorylates tyrosine 142 of histone H2AX and, therefore, it is thought to be involved in transcription regulation during organogenesis. Also, together with SIX1 and DACH2, EYA2 seems to be involved in myogensis. EYA2 translocation and transcriptional activity are inhibited by its direct interaction with activated GNAZ and GNAI2. EYA2 might be involved in the development of eye (1, 2).
1. Fan XM, et al., JBC, 275(4): 32129-32134, 2000. 2. The UniProt Consortium The Universal Protein Resource (UniProt) Nucleic Acids Res. 36:D190-D195(2008). UniProt ID: O0167
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