|Application ||WB, IHC|
|Calculated MW||69413 Da|
|Other Names||Ezrin, Cytovillin, Villin-2, p81, EZR, VIL2|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human Ezrin was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Ezrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.|
|Cellular Location||Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Note=Localization to the apical membrane of parietal cells depends on the interaction with MPP5 Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).|
|Tissue Location||Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.|
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Provided below are standard protocols that you may find useful for product applications.
Ezrin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton (1-2). Ezrin activity is regulated by intramolecular interactions between N- and C-terminal ERM association domains (3). Phosphorylation at threonine 567 is a critical regulator of ezrin function allowing the active protein to link target molecules to the actin cytoskeleton (4). Ezrin tyrosine phosphorylation can also be induced by EGF, PDGF and HGF stimulation. Ezrin interacts with PI3-K protein kinase A and Rho (5).
1. Bretscher, A., Edwards, K., and Fehon, R. G. (2002) Nat. Rev. Mol. Cell. Biol. 3, 586
2. Tsukita, S., and Yonemura, S. (1999) J. Biol. Chem. 274, 34507
3. Pearson, M. A., Reczek, D., Bretscher, A., and Karplus, P. A. (2000) Cell 101, 259-270
4. Gautreau, A., Louvard, D., and Arpin, M. (2000) J. Cell Biol. 150, 193-203
5. Bretscher, A., Chambers, D., Nguyen, R., and Reczek, D. (2000) Annu. Rev. Cell Dev. Biol. 16, 113
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