|Application ||WB, IHC|
|Calculated MW||83267 Da|
|Other Names||Coagulation factor XIII A chain, Coagulation factor XIIIa, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain, F13A1, F13A|
|Target/Specificity||A synthetic peptide corresponding to residues in human Factor XIII A was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Factor XIII Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.|
|Cellular Location||Cytoplasm. Secreted. Note=Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma|
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Provided below are standard protocols that you may find useful for product applications.
Factor XIII (plasma transglutaminase, fibrin stabilizing factor) is a plasma protein that plays an important role in the final stages of blood coagulation and fibrinolysis. It circulates in blood as a tetramer consisting of two A and two B subunits (1, 2). The amino acid sequence of the enzymatically active subunit, Factor XIII A, is unique and does not exhibit internal homology, but its active center is similar to that of the thiol proteases. Factor XIII A is activated by thrombin and calcium ion to a transglutaminase that catalyzes the cross linking of fibrin molecules, forming intermolecular isopeptide bonds, thus stabilizing blood clots (3, 4). In two diseases that share some histological resemblance (Lymphocyte-poor graft-versus-host-reaction and toxic epidermal necrolysis), Factor-XIII A-positive dendrocytes show some morphological changes, probably as a response to altered cytokine environment. Factor-XIII A-positive dendrocytes thus are reported to possibly play a role in the regulation of the connective tissue remodeling that may accompany epidermal destruction (5).
1. Ichinose A, et al. Biochemistry 25(22):6900-6, 1986
2. Ichinose A, et al. Proc Natl Acad Sci U S A. 5(16):5829-33, 1988
3. Takahashi N, et al. Proc. Nati. Acad. Sci. USA 83: 8019-8023, 1986
4. U Grundmann, et al. PNAS 83:8024-8028, 1986
5. T. Hermanns-L? et al. Dermatology 198:184-186, 1999
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