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FADD Antibody Phospho (pS194)

Rabbit Monoclonal Antibody

  • WB - FADD Antibody Phospho (pS194) AJ1284b
    A. Western blot analysis on Jurkat cell lysates using anti-phosho FADD (pS194) RabMAb (Cat. #AJ1284b), 1:500 dilution. Cells were either (A) untreated (B) treated with calyculin A
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q61160
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR1816Y
Calculated MW 22960 Da
Gene ID 14082
Other Names FAS-associated death domain protein, FAS-associating death domain-containing protein, Mediator of receptor induced toxicity, Protein FADD, Fadd, Mort1
Target/Specificity A synthetic peptide corresponding to residues surrounding serine 194 of human FADD was used as immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFADD Antibody Phospho (pS194) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Fadd
Synonyms Mort1
Function Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis (By similarity). Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling (By similarity).
Research Areas
Citations (0)

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Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1, and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1, which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8, which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also, the deficiency in FADD can inhibit TRAIL-induced cell apoptosis. In cancer cells, higher phopsholyation of FADD at serine 194 is expressed compared to normal cells. Phospholyation at serine 194 has been linked to induction of apoptosis by anti-cancer drugs in human protstate cancer cells and it is considered as a valuable maker for human prostate cancer progression (5).


1. Imtiyaz HZ et al, I J. Biol. Chem., 280(36);31360-67, 2005
2. Chinnaiyan AM et al. Cell 81, 505
3. Boldin MP et al, Cell 85, 803
4. Cohen PL et al. Annu. Rev. Immunol. 9, 243
5. Shimada K, et al. J Pathol 206:423-32, 2005.

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Cat# AJ1284b
(40 western blots)
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