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Fc-gamma (RIIb/CD32) Antibody Phospho-pY292

Rabbit Monoclonal Antibody

     
  • WB - Fc-gamma (RIIb/CD32) Antibody Phospho-pY292 AJ1287c
    A. Western blot analysis on Daudi cell lysates using anti-Phospho-Fc gamma RIIb (py292) RabMAb (Cat. #AJ1287c), 1:100,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P31994
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP926Y
Calculated MW 34044 Da
Gene ID 2213
Other Names Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b, CD32, FCGR2B, CD32, FCG2, IGFR2
Target/Specificity A phospho specific peptide corresponding to residues surrounding tyrosine 292 of human Fc gamma RIIb. This antibody detects Fc gamma RIIb phosphorylated on tyrosine 292.
Dilution WB~~1:50000~100000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFc-gamma (RIIb/CD32) Antibody Phospho-pY292 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FCGR2B
Synonyms CD32, FCG2, IGFR2
Function Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B- cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Is the most broadly distributed Fc-gamma- receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells
Research Areas
Citations (0)

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Background

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Coligation of FcgammaRIIb with the B-cell antigen receptor (BCR) results in the abrogation of B-cell activation and become phosphorlyated by protein tyrosine kinase (PTK) (4)

References

1. Ravetch J, et al. Annu Rev Immunol. 19: 275-90, 2001
2. Tsubata T. (1999). Curr Opin Immunol 11, 249
3. Malbec O.et al. Curr Microbiol Immunol 244, 13
4. Bewarder N, et al. Mol Cell Biol 16(9):4735-43, 1996

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Discontinued
Cat# AJ1287c
Size:
(40 western blots)
Alternative Products:

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