|Application ||WB, IHC|
|Calculated MW||51512 Da|
|Other Names||Fibrinogen gamma chain, FGG|
|Target/Specificity||A synthetic peptide corresponding to residues in human Fibrinogen gamma chain was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Fibrinogen gamma chain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re- epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3- dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.|
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Provided below are standard protocols that you may find useful for product applications.
Fibrinogen is comprised of two sets of three polypeptide chains termed alpha, beta and gamma that are joined by disulfide bridging within the N-terminal E domain (1). Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Fibrinogen binds through its gamma chain to cell surface receptors, growth factors, and coagulation factors to perform key roles in fibrin clot formation, platelet aggregation, and wound healing (2). Fibrinogen gamma chain, a fibrinogen gamma-chain variant generated via alternative mRNA processing, binding to thrombin and factor VIII results in clots that are mechanically stiffer and resistant to fibrinolysis (2). Elevated plasma fibrinogen gamma concentration is associated with myocardial infarction (3). Fibrinogen-gamma C-terminal fragments can induce endothelial barrier dysfunction and microvascular leak (4).
1. Mosesson et al. Ann N Y Acad Sci. 936: 11-30, 2001 2. Farrell DH. Current Opinion in Hematology. 11 (3):151- 155, 2004 3. Mannila et al. Journal of Thrombosis and Haemostasis. 5:766-773, 2007 4. Guo et al. Arterioscler. Thromb. Vasc. Biol. 29 (3):394-400, 2009
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