|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||280739 Da|
|Other Names||Filamin-A, FLN-A, Actin-binding protein 280, ABP-280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin, FLNA, FLN, FLN1|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human Filamin A was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Filamin-A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface- localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (By similarity).|
|Cellular Location||Cytoplasm, cell cortex. Cytoplasm, cytoskeleton|
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Provided below are standard protocols that you may find useful for product applications.
Filamins are a family of high molecular mass cytoskeletal proteins that organize filamentous actin in networks and stress fibers. Recently it has become clear that filamins anchor various transmembrane proteins to the actin cytoskeleton and provide a scaffold for a wide range of cytoplasmic signaling proteins (1). Actin-binding protein (Filamin A) is a ubiquitous dimeric actin cross-linking phosphoprotein of peripheral cytoplasm, where it promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins (2). The actin-binding domain is near the amino-terminus of the subunit where the amino acid sequence is similar to other actin filament binding proteins, including alpha-actinin, beta-spectrin, dystrophin, and Dictyostelium abp-120. A monoclonal antibody recognizing Filamin A and Fh1 binds to blood vessels, astrocytes, neurofibrillary tangles, neuropil threads, and dystrophic neurites in the AD brain. Detection of Filamin A /Fh1 proteins in these structures suggests that these presenilin-interacting proteins may be involved in the development of Alzheimer's disease and that interactions between presenilins and Filamin A /Fh1 may be functionally significant (3).
1. van der Flier A. Biochim Biophys 1538(2):99-117, 2001
2. Gorlin JB, et al. J Cell Biol 111(3):1089-105, 1990
3. Zhang W, et al. J Neurosci 18(3):914-22, 1998.
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