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Flotillin-1 Antibody

Rabbit Monoclonal Antibody

  • WB - Flotillin-1 Antibody AJ1303a
    A. Western blot analysis on K562 cell lysate using anti-Flotillin-1 RabMAb (Cat. #AJ1303a), dilution 1:500
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75955
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP446Y
Calculated MW 47355 Da
Gene ID 10211
Other Names Flotillin-1, FLOT1
Target/Specificity A synthetic peptide corresponding to residues in human Flotillin-1 was used as immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFlotillin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FLOT1
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Cellular Location Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome Endosome. Note=Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
Citations (0)

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Flotillin-1, also known as Reggie-2, belongs to a larger family of proteins that share an evolutionarily conserved stomatin/prohibitin/flotillin/HflK/C (SPFH) domain (1). Flotillin-1 is a lipid raft-associated protein that has been implicated in various cellular processes. Flotillin was first found in cells that lack morphologically definable caveolae, as well as in non-caveolar membranes (2) and was subsequently identified in low density detergent-insoluble complexes of endothelial cells, where they were termed flotillins (3). As well as its postulated role in neuronal regeneration, flotilllin-1 has been implicated in insulin signaling to trigger glucose transporter redistribution in adipocytes (4) Flotillin also associates with phagosomes in macrophages (5).


1. Tavernarakis, N., Driscoll, M., and Kyrpides, N. C. (1999) Trends Biochem. Sci. 24, 425
2. Stuermer, C. A., Lang, D. M., Kirsch, F., Wiechers, M., Deininger, S. O., and Plattner, H. (2001) Mol. Biol. Cell 12, 3031-3045
3. Bickel, P. E., Scherer, P. E., Schnitzer, J. E., Oh, P., Lisanti, M. P., and Lodish, H. F. (1997) J. Biol. Chem. 272, 13793-13802
4. Baumann, C. A., Ribon, V., Kanzaki, M., Thurmond, D. C., Mora, S., Shigematsu, S., Bickel, P. E., Pessin, J. E., and Saltiel, A. R. (2000) Nature 407, 202-207
5. Dermine, J. F., Duclos, S., Garin, J., St-, Louis, F., Rea, S., Parton, R. G., and Desjardins, M. (2001) J. Biol. Chem. 276, 18507-18512

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Cat# AJ1303a
(40 western blots)
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