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Flt3-L Antibody

Rabbit Monoclonal Antibody

  • WB - Flt3-L Antibody AJ1304a
    A. Western blot analysis on 10 uL recombinant protein using anti-Flt3-L RabMAb (Cat. #AJ1304a).
  • IHC - Flt3-L Antibody AJ1304a
    B. Immunohistochemical staining of paraffin-embedded human tonsils using anti-Flt3-LRabMAb (Cat. #AJ1304a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49771
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1140Y
Calculated MW 26416 Da
Gene ID 2323
Other Names Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG
Target/Specificity A synthetic peptide corresponding to residues within human Flt3-L was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFlt3-L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Human Flt3 ligand (Flt3L) stimulates early hematopoiesis by activating a type III tyrosine kinase receptor on primitive bone marrow stem cells. The crystal structure of soluble Flt3L reveals that it is a homodimer of two short chain alpha-helical bundles (1). Flt3 is a hematopoietic growth factor that stimulates cells via specific and structurally related tyrosine kinase receptors on the cell surface (2). This growth factor stimulated the proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow (3).


1. Savvides SN, et al. Nat Struct Biol. 7(6):486-91, 2000.
2. Lyman SD, et al. Oncogene 11(6):1165-72, 1995.
3. Lyman SD, et al. Blood. 83(10):2795-801, 1994.

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Cat# AJ1304a
(40 western blots)
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