|Application ||IHC, WB|
|Calculated MW||85387 Da|
|Other Names||FYN-binding protein, Adhesion and degranulation promoting adaptor protein, ADAP, FYB-120/130, p120/p130, FYN-T-binding protein, SLAP-130, SLP-76-associated phosphoprotein, FYB, SLAP130|
|Target/Specificity||A synthetic peptide corresponding to residues on the N-terminus of human FYB was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FYB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2. May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues|
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Provided below are standard protocols that you may find useful for product applications.
FYN-binding protein (FYB) is an adapter protein for Fyn and SLP-76 signaling cascades in T cells. A hematopoietic protein, FYB associates with p59fyn and becomes tyrosine-phosphorylated in response to receptor ligation. FYB has been associated to modulation of IL-2 expression in T cells and platelet activation (3). Fyn (p59Fyn) is a 59 kDa member of the Src family of tyrosine kinases. The carboxy terminus of Fyn shares extensive amino acid sequence homology with Src, but is very different within the amino-terminal 81 amino acid residues. The Fyn protein is synthesized and N-myristoylated on cytosolic polysomes and then rapidly targeted to the plasma membrane, where it is palmitoylated. Fyn's biological functions are diverse and include signaling via the T cell receptor, regulation of brain function and adhesion mediated signaling (2, 3).
1. Renzoni D.A., et al; Biochemistry 35:15646-1565, 1996.
2. Mustelin T, et al., Eur J Immunol. 1992 May;22(5):1173-8.
3. Silva AJ, et al. PNAS USA, 94(14):7493-7498, 1997.
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