|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||76616 Da|
|Other Names||GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1, GAB1|
|Target/Specificity||A synthetic peptide corresponding to residues in human Gab1 was used as immunogen. The antibody is predicted to detect splice isoform 2 based on sequence analysis.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Gab1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR).|
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Provided below are standard protocols that you may find useful for product applications.
Gab1 (Grb2-associated binder-1) is an adaptor protein that serves to activate numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs). Gab1 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors, and B cell antigen receptors; and it acts as a docking protein for several SH2-containing proteins (1-3). Tyrosine-phosphorylated Gab1 may recruit cytosolic signaling proteins to the cellular membrane, where they can act on membrane-bound targets as well as activate downstream pathways (4)
1. Holgado-Madruga, M., et al. A Grb2-associated docking protein in EGF- and insulin-receptor signalling. Nature 379: 560
2. Ingham, R.J., et al. The Gab1 docking protein links the b cell antigen receptor to the phosphatidylinositol 3-kinase/Akt signaling pathway and to the SHP2 tyrosine phosphatase. J. Biol. Chem. 276: 12257-12265 (2001)
3. Ingham, R.J., et al. The Gab1 protein is a docking site for multiple proteins involved in signaling by the B cell antigen receptor. J Biol Chem. 273: 30630
4. Schaeper, U., et al. Coupling of Gab1 to c-Met, Grb2, and Shp2 mediates biological responses. J Cell Biol. 149: 1419
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