|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||74458 Da|
|Other Names||GRB2-associated-binding protein 2, GRB2-associated binder 2, Growth factor receptor bound protein 2-associated protein 2, pp100, GAB2, KIAA0571|
|Target/Specificity||A synthetic peptide corresponding to residues in human Gab2 was used as immunogen. This antibody is predicted to detect the splice isoform 2 based on sequence homology.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Gab2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.|
|Cellular Location||Cytoplasm. Cell membrane|
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Provided below are standard protocols that you may find useful for product applications.
Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins (1-3). Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC (4-5).
1. Zhao, C.; Yu, D.-H.; Shen, R.; Feng, G.-S. : J. Biol. Chem. 274: 19649-19654, 1999.
2. Weiss A, Littman DR: Cell 76:263, 1994
3. Holgado-Madruga, M., Moscatello, D. K., Emlet, D. R., Dieterich, R., and Wong, A. J. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 12419-12424
4. Gu H, Pratt JC, Burakoff SJ, Neel B. Mol Cell. 1998;2:729-740
5. Nishida K, Yoshida Y, Itoh M, et al. Blood. 1999;93:1809-1816
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