|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||74458 Da|
|Other Names||GRB2-associated-binding protein 2, GRB2-associated binder 2, Growth factor receptor bound protein 2-associated protein 2, pp100, GAB2, KIAA0571|
|Target/Specificity||A synthetic peptide corresponding to residues in human Gab2 was used as immunogen. This antibody is predicted to detect the splice isoform 2 based on sequence homology.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Gab2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.|
|Cellular Location||Cytoplasm. Cell membrane|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins (1-3). Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC (4-5).
1. Zhao, C.; Yu, D.-H.; Shen, R.; Feng, G.-S. : J. Biol. Chem. 274: 19649-19654, 1999.
2. Weiss A, Littman DR: Cell 76:263, 1994
3. Holgado-Madruga, M., Moscatello, D. K., Emlet, D. R., Dieterich, R., and Wong, A. J. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 12419-12424
4. Gu H, Pratt JC, Burakoff SJ, Neel B. Mol Cell. 1998;2:729-740
5. Nishida K, Yoshida Y, Itoh M, et al. Blood. 1999;93:1809-1816
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.