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GAD2 Antibody

Rabbit Monoclonal Antibody

  • WB - GAD2 Antibody AJ1311a
    A. Western blot analysis on (A) fetal brain, (B) mouse brain and (C) rat brain lysates using anti-GAD2 RabMAb (Cat. #AJ1311a), dilution 1:1000.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q05329
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR2553Y
Calculated MW 65411 Da
Gene ID 2572
Other Names Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65
Target/Specificity A synthetic peptide corresponding to residues in human GAD2 was used as an immunogen.
Dilution WB~~1:500~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGAD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GAD2
Synonyms GAD65
Function Catalyzes the production of GABA.
Cellular Location Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters
Research Areas
Citations (0)

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Glutamate decarboxylase (GAD) catalyzes the production of gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter. GAD is represented by two isoforms, GAD1 and GAD2, which are the products of two different genes and differ substantially only at their N-terminal regions. In neurons and beta cells, GAD is concentrated around synaptic vesicles and synaptic-like microvesicles, respectively, as well as in the area of the Golgi complex. GAD2 lies primarily in axon terminals and only about half of GAD2 exists as active holoenzyme (1-2). The beta cell-specific marker GAD2 can be quantified in pancreatic cell extracts and in serum (3). GAD2 is a major target for autoantibodies and autoreactive T cells in patients with insulin-dependent diabetes mellitus. Autoantibodies to GAD are also found in patients with stiff man syndrome or polyendocrine autoimmunity (4).


1. M Solimena, et al. PNAS 90(7):3073-3077, 1993
2. Kaufman DL, et al. J Neurochem. 56(2):720-3, 1991
3. Rui M, et al. J Immunol Methods 19(1-2):133-43, 2007
4. Davenport C, et al. Clin Exp Immunol. 111(3):497-505, 1998

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Cat# AJ1311a
(40 western blots)
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