|Reactivity||Human, Mouse, Rat|
|Calculated MW||65411 Da|
|Other Names||Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65|
|Target/Specificity||A synthetic peptide corresponding to residues in human GAD2 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GAD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the production of GABA.|
|Cellular Location||Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters|
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Provided below are standard protocols that you may find useful for product applications.
Glutamate decarboxylase (GAD) catalyzes the production of gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter. GAD is represented by two isoforms, GAD1 and GAD2, which are the products of two different genes and differ substantially only at their N-terminal regions. In neurons and beta cells, GAD is concentrated around synaptic vesicles and synaptic-like microvesicles, respectively, as well as in the area of the Golgi complex. GAD2 lies primarily in axon terminals and only about half of GAD2 exists as active holoenzyme (1-2). The beta cell-specific marker GAD2 can be quantified in pancreatic cell extracts and in serum (3). GAD2 is a major target for autoantibodies and autoreactive T cells in patients with insulin-dependent diabetes mellitus. Autoantibodies to GAD are also found in patients with stiff man syndrome or polyendocrine autoimmunity (4).
1. M Solimena, et al. PNAS 90(7):3073-3077, 1993
2. Kaufman DL, et al. J Neurochem. 56(2):720-3, 1991
3. Rui M, et al. J Immunol Methods 19(1-2):133-43, 2007
4. Davenport C, et al. Clin Exp Immunol. 111(3):497-505, 1998
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