- CITATIONS: 1
|Reactivity||Human, Mouse, Rat|
|Calculated MW||24803 Da|
|Other Names||Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46, GAP43|
|Target/Specificity||A synthetic peptide corresponding to residues surrounding serine 41 of human GAP43 was used as an immunogen. This antibody detects both phosphorylated and nonphosphorylated GAP43.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GAP43 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.|
|Cellular Location||Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes|
Provided below are standard protocols that you may find useful for product applications.
Growth associated protein GAP43 (B-50, F1, neuromodulin) is a phosphoprotein of the presynaptic membrane involved in synaptic reorganization and neural development. Phospholyated at Ser41 by protein kinase C (PKC), phospholyated GAP43 has been correlated to release of neurotransmitters in mature nervous system (1). Also, GAP43 plays an essential role in the maintenance mechanism of long-term potentiation (LTP) (2). These suggest GAP43 to play a role in synaptic plasticity. For neurons in development or regeneration, GAP43 is a major component of the growth cone, which enables elongation and navigation of developing axons (3). During periods of low activity in neurons, Calmodulin binds to GAP43 when Ca2+ is low, suggesting Calmodulin as a negative regulator of GAP43 (4).
1. McIntosh H. et al. Visual Neuroscience. 2: 583-591, 1989.
2. Gianotti C, et al. Neuron 8:843-8, 1992.
3. Skene JH, et al. Science 233:783-786, 1986.
4. Neve RL, et al. J Neuro 18:7757-7767, 1998.
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