|Calculated MW||42751 Da|
|Other Names||Erythroid transcription factor, Eryf1, GATA-binding factor 1, GATA-1, GF-1, NF-E1 DNA-binding protein, GATA1, ERYF1, GF1|
|Target/Specificity||A synthetic peptide corresponding to N-terminus of human Gata-1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Gata1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).|
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Provided below are standard protocols that you may find useful for product applications.
Globin transcription factor 1 (GATA-1) is a transcription activator belonging to the GATA family of transcription factors with importance in cell growth. GATA-1 is a Cys2-Cys2 zinc finger DNA binding protein commonly found in erythroid red blood cells and megakaryotic platelet producing cells, and it is critical in the development process of both (1). It binds to the consensus sequence of [AT]GATA[AG] within regulatory regions of globin genes and genes in erythroid cells, leading to transcription of alpha spectrin structural protein necessary for the red blood cell's shape. In humans, GATA-1 can enhance the transcription rate up to 100 times (2). A mutation to the GATA-1 encoding gene has been implicated to X-linked dyserythropoietic anemia, thrombocytopenia, Down's syndrome in children, congenital transient myeloproliferative syndrome, and acute megakaryocytic leukemia (2, 3).
1. Osada H, et al., PNAS USA 92:9585-9, 1995.
2. Wong EY, et al., JBC 279:55024-33, 2004.
3. Wechsler J, et al., Nat Genet. 32: 148-52, 2002.
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