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GCN2 Antibody Phospho (pT899)

Rabbit Monoclonal Antibody

     
  • WB - GCN2 Antibody Phospho (pT899) AJ1318b
    A.Western blot analysis on HeLa cell lysates using anti-GCN2 phospho (pT899) RabMAb (Cat. #AJ1318b), 1:2000 dilution. Cells were either (A) untreated (B) treated with calyculin A
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9P2K8
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR2320Y
Calculated MW 186911 Da
Gene ID 440275
Other Names Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein, EIF2AK4, GCN2, KIAA1338
Target/Specificity A phospho specific peptide corresponding to residues surrounding threonine 899 of human GCN2 was used as an immunogen. This antibody detects GCN2 phosphorylyated at threonine 899.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGCN2 Antibody Phospho (pT899) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF2AK4 (HGNC:19687)
Synonyms GCN2, KIAA1338
Function Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:25329545). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF- 2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory. Plays a role in neurite outgrowth inhibition. Plays a proapoptotic role in response to glucose deprivation. Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress- activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q9QZ05}.
Tissue Location Widely expressed (PubMed:10504407). Expressed in lung, smooth muscle cells and macrophages (PubMed:24292273)
Research Areas
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Background

In eukaryotic cells, protein synthesis is regulated in response to various environmental stresses by phosphorylating the alpha subunit of the eukaryotic initiation factor 2 (eIF2alpha). Four different eIF2alpha kinases have been identified in mammalian cells, the heme-regulated inhibitor (HRI), the interferon-inducible RNA-dependent kinase (PKR) and the endoplasmic reticulum-resident kinase (PERK) and GCN2, which was previously characterized from Saccharomyces cerevisiae, Drosophila melanogaster and Neurospora crassa. GCN2 is the unique eIF2alpha kinase present in all eukaryotes from yeast to mammals (1). GCN2, a sensor of amino acid deficiency, plays a key role in yeast and mammals in modulating amino acid metabolism as part of adaptation to nutrient deprivation. (2). Results demonstrate that PERK and GCN2 function to cooperatively regulate eIF2alpha phosphorylation and cyclin D1 translation after unfolded protein response pathway activation (3).

References

1. Berlanga JJ, et al. Eur J Biochem 265(2):754-62
2. Guo F, et al. Cell Metab 5(2):103-14, 2007
3. Hamanaka RB, et al. Mol Bio Cell 16(12):5493-501, 2005

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Discontinued
Cat# AJ1318b
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