|Application ||IHC, WB|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||85698 Da|
|Other Names||Gelsolin, AGEL, Actin-depolymerizing factor, ADF, Brevin, GSN|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-terminal region of human Gelsolin was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Gelsolin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.|
|Cellular Location||Isoform 2: Cytoplasm, cytoskeleton.|
|Tissue Location||Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells|
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Provided below are standard protocols that you may find useful for product applications.
Gelsolin is a large and complex actin-binding protein that belongs to actin-severing gelsolin/villin super family. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).
1. Sun, H et al. J Biol Chem 274 (47): 33179
2. Koya, R, et al. J Biol Chem 275 (20): 15343-15349, 2000.
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