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GFAP Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - GFAP Antibody (C-term) AJ1320a
    A. Western blot analysis on Rat brain lysate using anti-GFAP (C-term) RabMAb (Cat. #AJ1320a), dilution 1:2,000.
  • IHC - GFAP Antibody (C-term) AJ1320a
    B. Immunohistochemical analysis of paraffin-embedded human brain using anti-GFAP (C-term) RabMAb (Cat. #AJ1320a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14136
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP672Y
Calculated MW 49880 Da
Gene ID 2670
Other Names Glial fibrillary acidic protein, GFAP, GFAP
Target/Specificity A synthetic peptide corresponding to residues in the C-term of human GFAP was used as immunogen.
Dilution WB~~1:5000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGFAP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Cellular Location Cytoplasm. Note=Associated with intermediate filaments
Tissue Location Expressed in cells lacking fibronectin.
Research Areas
Citations (0)

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Glial fibrillary acidic protein (GFAP) belongs to the class III of the intermediate filament (IF) proteins highly specific to astrocytes which function as nerve cells in the brain and in the central nervous system (CNS) (1-2). Two other class III IF proteins, nestin and vimentin, dominate in immature astrocytes, while GFAP is most abundant in mature astrocytes (3). GFAP is used as a marker to distinguish astrocytes form other glial cells during development. Mutations in GFAP cause Alexander disease, a rare disorder of the CNS. Most frequent mutations are located at Arginine 79 or 239 (4).


1. Reeves, S. A.; Helman, L. J.; Allison, A.; Israel, M. A. : Proc. Nat. Acad. Sci. 86: 5178-5182, 1989.
2. Fuchs, E., and Weber, K. (1994) Annu. Rev. Biochem. 63, 345-382
3. Bongcam-Rudloff, E., Nister, M., Betsholtz, C., Wang, J. L., Stenman, G., Huebner, K., Croce, C. M., and Westermark, B. (1991) Cancer Res. 51, 1553-1560
4. Li et all. Ann. Neurol. 57: 310-326, 2005.

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Cat# AJ1320a
(40 western blots)
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