|Application ||WB, IHC|
|Calculated MW||49880 Da|
|Other Names||Glial fibrillary acidic protein, GFAP, GFAP|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-term of human GFAP was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GFAP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.|
|Cellular Location||Cytoplasm. Note=Associated with intermediate filaments|
|Tissue Location||Expressed in cells lacking fibronectin.|
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Provided below are standard protocols that you may find useful for product applications.
Glial fibrillary acidic protein (GFAP) belongs to the class III of the intermediate filament (IF) proteins highly specific to astrocytes which function as nerve cells in the brain and in the central nervous system (CNS) (1-2). Two other class III IF proteins, nestin and vimentin, dominate in immature astrocytes, while GFAP is most abundant in mature astrocytes (3). GFAP is used as a marker to distinguish astrocytes form other glial cells during development. Mutations in GFAP cause Alexander disease, a rare disorder of the CNS. Most frequent mutations are located at Arginine 79 or 239 (4).
1. Reeves, S. A.; Helman, L. J.; Allison, A.; Israel, M. A. : Proc. Nat. Acad. Sci. 86: 5178-5182, 1989.
2. Fuchs, E., and Weber, K. (1994) Annu. Rev. Biochem. 63, 345-382
3. Bongcam-Rudloff, E., Nister, M., Betsholtz, C., Wang, J. L., Stenman, G., Huebner, K., Croce, C. M., and Westermark, B. (1991) Cancer Res. 51, 1553-1560
4. Li et all. Ann. Neurol. 57: 310-326, 2005.
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