|Calculated MW||26886 Da|
|Other Names||Green fluorescent protein, GFP|
|Target/Specificity||A synthetic peptide corresponding to residues in the N-terminus of Aequorea victoria green fluorescent protein was used as immunogen. The antibody recognizes GFP and GFP fusion proteins.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GFP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.|
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Provided below are standard protocols that you may find useful for product applications.
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria (1) is extensively used as a fluorescent marker in molecular and cell biology both in vitro and in vivo. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. This motif, with beta-structure on the outside and alpha-helix on the inside, is also referred as the beta-can (2-3). This rabbit monoclonal antibody can recognize wild type as well as the humanized formed of GFP and is especially useful for working with GFP expressed in Rodent cell lines or tissues.
1. Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J.; Primary structure of the Aequorea victoria green-fluorescent protein.; Gene 111:229-233(1992).
2. Cody C.W., Prasher D.C., Westler W.M., Prendergast F.G., Ward W.W.; Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.; Biochemistry 32:1212-1218(1993).
3. Ormoe M., Cubitt A.B., Kallio K., Gross L.A., Tsien R.Y., Remington S.J.; Crystal structure of the Aequorea victoria green fluorescent protein.; Science 273:1392-1395(1996).
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