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GM130 Antibody

Rabbit Monoclonal Antibody

  • WB - GM130 Antibody AJ1327a
    A. Western blot analysis on HeLa cell lysate using anti-GM130 Rabbit Monoclonal (Cat. #AJ1327a), dilution 1:20,000
  • IHC - GM130 Antibody AJ1327a
    B. Immunohistochemical analysis of paraffin-embedded human liver using anti-Golgi matrix protein RabMAb, (Cat. #AJ1327a)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q08379
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP892Y
Calculated MW 113086 Da
Gene ID 2801
Other Names Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, GOLGA2
Target/Specificity A synthetic peptide corresponding to residues near the N-term of human Golgi Matrix Protein (GM130) was used as a immunogen.
Dilution WB~~1:50000~100000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGM130 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Cellular Location Golgi apparatus, Golgi stack membrane; Peripheral membrane protein
Research Areas
Citations (0)

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Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.


1. Nakamura N, et al. J Cell Biol 131:1715-1726, 1995
2. Warren G, Annual Rev Biochem 62:323-348, 1993
3. Weide T, et al. EMBO Rep 2:336-341, 2001

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Cat# AJ1327a

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