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LGR5/GPR49 Antibody

Rabbit Monoclonal Antibody

  • WB - LGR5/GPR49 Antibody AJ1330a
    A.Western blot analysis on fetal skeletal muscle lysate using anti-LGR5/GPR49 RabMAb (Cat. #AJ1330a), dilution 1:1000.
  • IHC - LGR5/GPR49 Antibody AJ1330a
    B.Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-LGR5/GPR49 RabMAb (Cat. #AJ1330a).
  • IHC - LGR5/GPR49 Antibody AJ1330a
    C.Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using anti-LGR5/GPR49 RabMAb (Cat. #AJ1330a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75473
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3065Y
Calculated MW 99998 Da
Gene ID 8549
Other Names Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
Target/Specificity A synthetic peptide corresponding to residues in human GPR49 was used as an immunogen.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLGR5/GPR49 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LGR5
Synonyms GPR49, GPR67
Function Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Cellular Location Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).
Tissue Location Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver
Research Areas
Citations (0)

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G-protein coupled receptor GPR49 (also known as Leucine-rich repeat containing G-protein coupled receptor 5, Lgr5) is closely related to members of the glycoprotein hormone receptor subfamily. It is expressed in skeletal muscle, placenta, spinal cord, and various regions of the brain (1). GPR49 is reported to possibly mark stem cells in multiple adult tissues and is over expressed in cancers (2).


1. McDonald T, et al. Biochem Biophys Res Commun. 247(2):266-70, 1998
2. Barker N, Nature. 449(7165):1003-7, 2007
3. Tanese K, et al. Am J Pathol. 173(3):835-43, 2008
4. McClanahan T, et al. Cancer biology & therapy 5(4):419-426, 2006

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Cat# AJ1330a
(40 western blots)
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