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GRB2 Antibody (N-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1331b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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GRB2 Antibody (N-term) - Product info | |
| Application | WB, IHC, IF
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| Primary Accession | P62993 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | Y301 |
| Calculated MW | 25206 Da |
| Gene ID 2885 | |
| Other Names GRB2, ASH, Growth factor receptor-bound protein 2, Adapter protein GRB2;SH2/SH3 adapter GRB2;Protein Ash | |
| Target/Specificity A synthetic peptide corresponding to residues in the N-terminus of human GRB2 was used as an immunogen. This antibody can also detect the splice isoform GRB3-3 of GRB2. | |
| Dilution WB~~1:1000~10000 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions GRB2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
GRB2 Antibody (N-term) - Protein Information | |
| Name GRB2 | |
| Synonyms ASH | |
| Function Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway | |
| Cellular Location Nucleus. Cytoplasm. Endosome. Golgi apparatus (By similarity) | |
GRB2 Antibody (N-term) - Related products
AP6283b: GRB2 Antibody (N-term)
AP6283c: GRB2 Antibody (Center)
LY11018a: GRB2 Over-expression Lysate
BP6283a: GRB2 Antibody (Y209) Blocking Peptide
BP6283b: GRB2 Antibody (N-term) Blocking Peptide
BP6283c: GRB2 Antibody (Center) Blocking Peptide
AJ1331a: GRB2 Antibody (C-term)
GRB2 Antibody (N-term) - Application data
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A. Western blot analysis of anti-GRB2 (N-term) RabMAb (Cat. #AJ1331b), dilution 1:500 A-NIH 3T3 B-PC12 C-Hela
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B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-GRB2 (N-term) RabMAb (Cat. #AJ1331b).
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C. Immunofluorescent staining of PC-12 cells using anti-GRB-2 (N-term) RabMAb (Cat. #AJ1331b).
GRB2 Antibody (N-term) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).
REFERENCES
1. Matuoka, K.; Shibata, M.; Yamakawa, A.; Takenawa, T.: Proc. Nat. Acad. Sci. 89: 9015-9019, 1992
2. Lowenstein, E. J.; Daly, R. J.; Batzer, A. G.; Li, W.; Margolis, B.; Lammers, R.; Ullrich, A.; Skolnik, E. Y.; Bar-Sagi, D.; Schlessinger, J.: Cell 70: 431-442, 1992.
3. Nioche P.; et al, JMB, Volume 315, Number 5, February ( 2002), 1167-1177(11)
