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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GRP1 Antibody   

GRP1 Antibody

Rabbit Monoclonal Antibody

     
  • WB - GRP1 Antibody AJ1333a
    A. Western blot analysis on Hunan brain lysate using anti-GRP1 RabMAb (Cat. #AJ1333a).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O43739
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP394Y
Calculated MW 46349 Da
Gene ID 9265
Other Names Cytohesin-3, ARF nucleotide-binding site opener 3, Protein ARNO3, General receptor of phosphoinositides 1, Grp1, PH, SEC7 and coiled-coil domain-containing protein 3, CYTH3, ARNO3, GRP1, PSCD3
Target/Specificity A synthetic peptide corresponding to residues near the SEC7 domain of GRP1 was used as immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGRP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYTH3
Synonyms ARNO3, GRP1, PSCD3
Function Promotes guanine-nucleotide exchange on ARF1 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP.
Cellular Location Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Note=Translocates from the cytosol to membranes enriched in phosphatidylinositol 3,4,5-trisphosphate
Tissue Location Almost absent from liver, thymus and peripheral blood lymphocytes
Research Areas
Citations (0)

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Background

GRP1 (general receptor for 3-phosphoinositides) is composed of an N-terminal heptad repeat, an Arf exchange domain with homology to the yeast Sec7 protein, a PH domain, and a C-terminal polybasic region (1). The PH domain of GRP1 binds PtdIns(3, 4, 5)P3 with high affinity, but not PtdIns(3, 4)P2 or PtdIns(3)P, potentially making it a target for PI-3-kinase. Furthermore, GRP1 acts as a nucleotide exchange factor for the small GTP proteins of the ARF family (2). While GRP1 catalyses guanine-nucleotide exchange specifically on ARF5; other GRP1 homologues ( ARNO and cytohesin-1) will only function on ARF6 and ARF3 respectively (3).

References

1. Klarlund J, Guilherme A, Holik JJ, Virbasius J, V, Chawla A, Czech MP 1997 Science 275:1927
2. Klarlund et al. J Biol Chem 273:1859
3. Klarlund, J. K., Rameh, L. E., Cantley, L. C.,Buxton, J. M., Holik, J. J., Sakelis, C., Patki, V., Corvera, S. and Czech,M. P. (1998) J. Biol. Chem. 273, 1859

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Discontinued
Cat# AJ1333a
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