|Reactivity||Human, Mouse, Rat|
|Calculated MW||46349 Da|
|Other Names||Cytohesin-3, ARF nucleotide-binding site opener 3, Protein ARNO3, General receptor of phosphoinositides 1, Grp1, PH, SEC7 and coiled-coil domain-containing protein 3, CYTH3, ARNO3, GRP1, PSCD3|
|Target/Specificity||A synthetic peptide corresponding to residues near the SEC7 domain of GRP1 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GRP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ARNO3, GRP1, PSCD3|
|Function||Promotes guanine-nucleotide exchange on ARF1 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP.|
|Cellular Location||Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Note=Translocates from the cytosol to membranes enriched in phosphatidylinositol 3,4,5-trisphosphate|
|Tissue Location||Almost absent from liver, thymus and peripheral blood lymphocytes|
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Provided below are standard protocols that you may find useful for product applications.
GRP1 (general receptor for 3-phosphoinositides) is composed of an N-terminal heptad repeat, an Arf exchange domain with homology to the yeast Sec7 protein, a PH domain, and a C-terminal polybasic region (1). The PH domain of GRP1 binds PtdIns(3, 4, 5)P3 with high affinity, but not PtdIns(3, 4)P2 or PtdIns(3)P, potentially making it a target for PI-3-kinase. Furthermore, GRP1 acts as a nucleotide exchange factor for the small GTP proteins of the ARF family (2). While GRP1 catalyses guanine-nucleotide exchange specifically on ARF5; other GRP1 homologues ( ARNO and cytohesin-1) will only function on ARF6 and ARF3 respectively (3).
1. Klarlund J, Guilherme A, Holik JJ, Virbasius J, V, Chawla A, Czech MP 1997 Science 275:1927
2. Klarlund et al. J Biol Chem 273:1859
3. Klarlund, J. K., Rameh, L. E., Cantley, L. C.,Buxton, J. M., Holik, J. J., Sakelis, C., Patki, V., Corvera, S. and Czech,M. P. (1998) J. Biol. Chem. 273, 1859
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