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GSK3 Antibody alpha/beta Phospho (pY279)/(pY216)

Rabbit Monoclonal Antibody

  • WB - GSK3 Antibody alpha/beta Phospho (pY279)/(pY216) AJ1334d
    A. Western blot analysis on 293 cell lysates using anti-Phospho-GSK3a/b (pY279/pY216) RabMAb (Cat. #AJ1334d), 1:2000 dilution. Cells were either (A) untreated (B) treated with AP
  • IHC - GSK3 Antibody alpha/beta Phospho (pY279)/(pY216) AJ1334d
    B. Immunohistochemical analysis of parafffin-embedded human brain using anti-GSK3aGSK3b (pY279)/(pY216) RabMAb (Cat. #AJ1334d).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49840
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR933Y
Calculated MW 50981 Da
Gene ID 2931
Other Names Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A, GSK3A
Target/Specificity A phospho specific peptide corresponding to residues surrounding tyrosine 279 in human GSK3a and tyrosine 216 in human GSKb was used as an immunogen. This antibody detects both GSKα phosphorylated on tyrosine 279 and GSK3β phosphorylyated on tyrosine 216.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSK3 Antibody alpha/beta Phospho (pY279)/(pY216) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSK3A
Function Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.
Research Areas
Citations (0)

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Glycogen synthases kinase 3 alpha (GSK3-α) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-α activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-α by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-α activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4). GSK3 beta) plays a key role in insulin and Wnt signaling, phosphorylating downstream targets by default, and becoming inhibited following the extracellular signaling event (5).


1. Martinho RG, et al. EMBO J. 17:7239-7249, 1998
2. Sanchez Y, et al. Science 277:1497-1501, 1997
3. Lovestone S, et al. Curr Biol 4(12):1077-1086, 1994
4. Dajani R, et al. Cell 105(6): 721-32

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Cat# AJ1334d
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