|Application ||WB, IHC, IF|
|Calculated MW||25497 Da|
|Other Names||Glutathione S-transferase kappa 1, GST 13-13, GST class-kappa, GSTK1-1, hGSTK1, Glutathione S-transferase subunit 13, GSTK1|
|Target/Specificity||A synthetic peptide corresponding to residues in human Gstk1was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Gstk1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).|
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Provided below are standard protocols that you may find useful for product applications.
Glutathione transferases (Gst) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells. The kappa class (Gstk) is the least studied one among various classes within the superfamily. The TRX-like domain of Gstk shares 19% sequence identity and structure similarity with the theta class Gst, suggesting that the kappa class is more closely related to the theta class enzyme within the Gst superfamily (1). Research has demonstrated that Gstk1 is expressed at relatively high levels in liver and stomach, and at moderate levels in kidney, heart, large intestine, testis, and lung. Sparse or essentially no Gstk1 protein was reported to be detected in small intestine, brain, spleen, and skeletal muscle (2).
1. Li J, et al. Protein Sci, 14(9):2361-9, 2005
2. Thomson RE, et al. J Histochem Cyto 52(5):653-62, 2004
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