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HER2 Antibody Phospho (pT1221/T1222)

Rabbit Monoclonal Antibody

  • WB - HER2 Antibody Phospho (pT1221/T1222) AJ1340e
    Western blot analysis on SKBR3 cell lysates using anti-Phospho-HER2 (pT1221/T1222) RabMAb (Cat. #AJ1340e), 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda phosphatase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04626
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR1047
Calculated MW 137910 Da
Gene ID 2064
Other Names Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340, ERBB2, HER2, MLN19, NEU, NGL
Target/Specificity A phosphor-specific peptide corresponding to residues surrounding tyrosine 1222 of human HER2 was used as an immunogen. This antibody detects HER2 phosphorylated at tyrosine 1222.
Dilution WB~~1:5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHER2 Antibody Phospho (pT1221/T1222) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERBB2
Synonyms HER2, MLN19, NEU, NGL
Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1 Isoform 3: Cytoplasm. Nucleus.
Tissue Location Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
Research Areas
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HER2 (ErbB2, epidermal growth factor receptor 2) is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of HER2 can be activated without ligand if it is overexpressed, and by association with other HER proteins (2). Overexpression of HER2 is detected in almost 40% of human breast cancers (3). HER2 is considered one of the major targets for the treatment of breast cancer and other carcinomas. Binding of c-Cbl ubiquitin ligase to Tyr1112 of HER2 leads to poly-ubiquitination of HER2 and enhances its degradation (4). Similar to other growth factors, HER2 exhibits intrinsic protein tyrosine kinase activity and undergoes autophosphorylation (Y1023, Y1248, Y1139, and Y1222). A phosphorylation at tyrosine 1139 has been identified to induce the binding of Src to HER2, leading to HER2 activation of Stat3 alpha (5).


1. Muthuswamy, S. K., et al. Mol. Cell. Biol. 19: 6845-6857 (1999). 2. Qian, X., et al. Proc. Natl. Acad. Sci. USA 91: 1500 3. Montgomery RB, Cancer Res 65(2):650-6, 2005 4. Klapper, L. N., et al. Cancer Res. 60: 3384-3388 (2000). 5. Segatto O, et al. New Biol 2(2):187-95, 1990

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Cat# AJ1340e
(40 western blots)
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