|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||55364 Da|
|Other Names||Histone deacetylase 2, HD2, HDAC2|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-term of human Histone Deacetylase 2 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Histone Deacetylase 2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Widely expressed; lower levels in brain and lung|
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Provided below are standard protocols that you may find useful for product applications.
Histone deacetylase 2 (HDAC2) belongs to the histone deacetylase family type 1 which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (1). HDAC2 represses transcription and binds transcription factor YY1 to form a large complex (2). It also forms transcriptional repressor complexes by associating with MAD, SIN3, N-CoR and DNMT1 (3-4). Similar to HDAC1 and HDAC3, HDAC2 does not possess a repression domain, but preferentially associates with HDAC1. HDAC2 with HDAC1 is also implicated in a complex of multiproteins that functions through modifying chromatin structure to keep genes silent (5).
1. Yang, W.-M.; Inouye, C.; Zeng, Y.; Bearss, D.; Seto, E.: Proc. Nat. Acad. Sci. 93: 12845-12850, 1996.
2. Inouye, C. J.; Seto, E.: J. Biol. Chem. 269: 6506-6510, 1994.
3. Tong, J. K., Hassig, C. A., Schnitzler, G. R., Kingston, R. E. & Schreiber, S. L. (1998) Nature (London) 395, 917-921
4. Rountree, M. R.; Bachman, K. E.; Baylin, S. B.: Nature Genet. 25: 269-277, 2000.
5. Hakimi et al. J. Biol. Chem. 278: 7234-7239, 2003
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