|Application ||WB, IHC|
|Calculated MW||48848 Da|
|Other Names||Histone deacetylase 3, HD3, RPD3-2, SMAP45, HDAC3|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of Histone deacetylase 3 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Histone Deactylase-3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys- 27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (PubMed:21444723, PubMed:23911289). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed:25190803).|
|Cellular Location||Nucleus. Cytoplasm. Cytoplasm, cytosol. Note=Colocalizes with XBP1 and AKT1 in the cytoplasm (PubMed:25190803). Predominantly expressed in the nucleus in the presence of CCAR2.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Histone deacetylase 3 belongs to the histone deacetylase family type 1 which actively deacetylate Histones. Currently 3 known isoforms exists (HDAC3, HDAC3A and HDAC3C) (1). HDAC3 represses transcription and binds transcription factor YY1 to form a large complex. N-Cor binds HDAC3 in a different manner than other HDACs. In addition to N-Cor, HDAC3 will form a complex with corepressor SMRT (2). HDAC3 is also known to interact with DAXX, HDAC10 and DACH1.
1. Wen-Ming Yang, et al. 272, (44), 28001-28007, 1997
2. Li, J., et al, EMBO J. 19, pp. 4342
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