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Histone H2A.x Antibody Phospho (pS139)

Rabbit Monoclonal Antibody

  • WB - Histone H2A.x Antibody Phospho (pS139) AJ1351a
    A. Western blot analysis on Jurkat cell lysates using anti-Phospho-H2A.x (pS139) RabMAb (Cat. #AJ1351a). Cells were either (A) untreated (B) treated with etoposide
  • IHC - Histone H2A.x Antibody Phospho (pS139) AJ1351a
    B. Immunohistochemical analysis of paraffin-embedded human kidney transitional cell carcinoma using anti-Histone H2A.x RabMAb (Cat. #AJ1351a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16104
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP854(2)Y
Calculated MW 15145 Da
Gene ID 3014
Other Names Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding serine 139 of human H2A.x protein.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHistone H2A.x Antibody Phospho (pS139) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2AFX
Synonyms H2AX
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
Cellular Location Nucleus Chromosome
Research Areas
Citations (0)

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As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair (1). As a part of post-translational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis (2)


1. Crowe, S, et al; Eur J Neurosci. 23(9): 2351
2. Song, X, et al; Mol Cell Biol. 27(7): 2648

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Cat# AJ1351a
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