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Histone H2B AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1352b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
Histone H2B Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q5QNW6 |
| Reactivity | Human, Mouse |
| Clone Names | EP819Y |
| Calculated MW | 13920 Da |
| Gene ID 440689 | |
| Other Names HIST2H2BF, Histone H2B type 2-F | |
| Target/Specificity A synthetic peptide corresponding to residues of human Histone H2B was used as an immunogen. | |
| Dilution WB~~1:10000 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Histone H2B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Histone H2B Antibody - Protein Information | |
| Name HIST2H2BF | |
| Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling | |
| Cellular Location Nucleus. Chromosome. | |
Histone H2B Antibody - Related products
AP10223b: HIST2H2BF Antibody (C-term)
BP10223b: HIST2H2BF Antibody (C-term) Blocking peptide
AJ1346a: Histon H2B Antibody Type 1B
AJ1356a: Histone-H2B Antibody Acetyl (K12)
AJ1356b: Histone-H2B Antibody Acetyl (K15)
Histone H2B Antibody - Application data
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A. Western blot analysis on A431 cell lysate using anti-H2B Rabbit Monoclonal (Cat. #AJ1352b), dilution 1:10,000.
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B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone H2B RabMAb(Cat. #AJ1352b)
Histone H2B Antibody - Research Areas
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BACKGROUND
Changes in chromatin structure play a key role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is acetylated in the N-terminal region, primary at lysines 5, 12, 15, and 20 (3). Ubiquitylation of histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of cell apoptosis and meiosis (5).
REFERENCES
1. Braunstein M, et al. Mol. Cell. Biol. 16:4349
2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545
3. Cheung, P. et al. Cell103, 263
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005.
5. Ahn SH, et al. Cell Cycle 4:780-783, 2005.
