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Histone H2B Antibody

Rabbit Monoclonal Antibody

  • WB - Histone H2B Antibody AJ1352c
    A. Western blot analysis on A431 cell lysate using anti-Histone H2B RabMAb (Cat. #AJ1352c).
  • IHC - Histone H2B Antibody AJ1352c
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone H2B RabMAb (Cat. #AJ1352c)
  • IF - Histone H2B Antibody AJ1352c
    C. Fluorescent staining of HeLa cells using anti-Histone H2B RabMAb (Cat. #AJ1352c).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5QNW6
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP957Y
Calculated MW 13920 Da
Gene ID 440689
Other Names Histone H2B type 2-F, HIST2H2BF
Target/Specificity A synthetic acetylated peptide corresponding to residues surrounding Histone H2B was used as an immunogen.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHistone H2B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)

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Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).


1. Braunstein M, et al. Mol. Cell. Biol. 16:4349
2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545
3. Cheung, P. et al. Cell103, 263
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005.
5. Ahn SH, et al. Cell Cycle 4:780-783, 2005.

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Cat# AJ1352c
(40 western blots)
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