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Histone H3 Antibody Acetyl (K14)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1353b | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Histone H3 Antibody Acetyl (K14) - Product info | |
| Application | WB, IHC, IF
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| Primary Accession | P68431 |
| Reactivity | Human, Rat |
| Clone Names | EP964Y |
| Calculated MW | 15404 Da |
| Gene ID 8968 | |
| Other Names HIST1H3J, H3FJ, Histone H3.1, H3/a;H3/b;H3/c;H3/d;H3/f;H3/h;H3/i;H3/j;H3/k;H3/l | |
| Target/Specificity A synthetic acetyl-specific peptide corresponding to residues around Lysine 14 was used as an immunogen. This antibody recognizes Histone H3 acetylated on K14. | |
| Dilution WB~~1:500 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Histone H3 Antibody Acetyl (K14) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Histone H3 Antibody Acetyl (K14) - Protein Information | |
| Name HIST1H3A | |
| Synonyms H3FA | |
| Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling | |
| Cellular Location Nucleus. Chromosome. | |
Histone H3 Antibody Acetyl (K14) - Related products
AP3003a: Phospho-H3-S10 Antibody
AP3004a: Phospho-H3-S10 Antibody
AP3007a: Phospho-H3-S28 Antibody
AP3115a: Phospho-HIST1H3B3-S10 Antibody
RI12466: HIST1H3A predesign siRNA
RI12467: HIST1H3B predesign siRNA
RI12468: HIST1H3C predesign siRNA
RI12469: HIST1H3D predesign siRNA
RI12470: HIST1H3E predesign siRNA
RI12471: HIST1H3F predesign siRNA
RI12472: HIST1H3G predesign siRNA
RI12473: HIST1H3H predesign siRNA
RI12474: HIST1H3I predesign siRNA
RI12475: HIST1H3J predesign siRNA
LY12040a: HIST1H3C Over-expression Lysate
BP3003a: Phospho-H3-S10 Antibody Blocking Peptide
BP3004a: Phospho-H3-S10 Antibody Blocking Peptide
BP3007a: Phospho-H3-S28 Antibody Blocking Peptide
BP3115a: Phospho-HIST1H3B3-pS10 Antibody Blocking Peptide
AT2374a: HIST1H3D Antibody (monoclonal) (M01)
AJ1353b: Histone H3 Antibody Acetyl (K14)
AJ1353e: Histone H3 Antibody Phospho (pT3)
AJ1357a: Histone-H3 Antibody Acetyl (K18)
AJ1357b: Histone-H3 Antibody Acetyl (K56)
AJ1359a: Histone-H3T Antibody Acetyl (K27)
Histone H3 Antibody Acetyl (K14) - Application data
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A. Western blot analysis on C6 cell lysates using anti- Histone H3TB Acetyl K14 RabMAb (Cat. #AJ1353b), 1:2000 dilution. Cells were either (A) untreated (B) treated with TSA.
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B. Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of uterus using anti Histone H3TBRabMAb(Cat. #AJ1353b).
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C. Immunofluorescent staining of HeLa cells using anti-Histone H3TB RabMAb (Cat. #AJ1353b).
Histone H3 Antibody Acetyl (K14) - Research Areas
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BACKGROUND
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). . H3t (H.34) differs from the consensus mammalian H3 structure by four amino acid residues and it is mainly expressed in testicular cells. H3t encoding gene maps to chromosome 1 (1q42), unlike the majority of the human histone genes that form cluster on chromosome 6 and outside of replication-dependent H3 genes (5).
REFERENCES
1. Braunstein M, et al. Mol. Cell. Biol. 16:4349
2. Workman JL, et al. Annu. Rev. Biochem. 67: 545
3. Cheung, P. et al. Cell 103, 263
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005
5. Albig W, et al. Hum Genet 97:486-491. 1996
