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Histone H3 Antibody Phospho (pS10)

Rabbit Monoclonal Antibody

  • WB - Histone H3 Antibody Phospho (pS10) AJ1353c
    A. Western blot analysis on HeLa cell lysate using anti-Phospho-Histone H3 (pS10) RabMAb (Cat. #AJ1353c), 1:2,500 dilution. Cell lysates were either (1) untreated or (2) treated with Alkaline Phosphatase.
  • IHC - Histone H3 Antibody Phospho (pS10) AJ1353c
    B. Immunohistochemical analysis of paraffin-embedded lymphoma using anti-Phospho-Histone H3 (pS10) RabMAb (Cat. #AJ1353c).
  • IF - Histone H3 Antibody Phospho (pS10) AJ1353c
    C. Immunofluorescent staining of Hela cells using anti-Phospho-Histone H3 (pS10) RabMab(Cat. #AJ1353c).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16695
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E173
Calculated MW 15508 Da
Gene ID 8290
Other Names Histone H31t, H3/t, H3t, H3/g, HIST3H3, H3FT
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 10. Predicted to cross-react with most species, based on sequence homology.
Dilution WB~~1:200000~1000000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHistone H3 Antibody Phospho (pS10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST3H3
Synonyms H3FT
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Tissue Location Expressed in testicular cells.
Research Areas
Citations (0)

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Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5).


1. Braunstein, M., et al. Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern. Mol. Cell. Biol. 16: 4349
2. Workman, J.L. and R.E. Kingston. Alteration of nucleosome structure as a mechanism of transcriptional regulation. Annu. Rev. Biochem. 67: 545
3. Hansen, J.C. et al. Structure and function of the core histone N-termini: more than meets the eye. Biochemistry 37, 17637
4. Strahl, B.D. and C.D. Allis. The language of covalent histone modifications. Nature 403, 41
5. Hendzel, M.J., et al. Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106: 348

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Cat# AJ1353c
(40 western blots)
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