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Histone H3.3 Antibody Phospho (pS31)Rabbit Monoclonal
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1354a | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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Histone H3.3 Antibody Phospho (pS31) - Product info | |
| Application | WB, IHC, IF
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| Primary Accession | P84243 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EPR1873 |
| Calculated MW | 15328 Da |
| Gene ID 3021 | |
| Target/Specificity A synthetic peptide corresponding to residues surrounding Serine 31 of human Histone H3.3 was used as an immunogen. | |
| Dilution WB~~1:1000~5000 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Histone H3.3 Antibody Phospho (pS31) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Histone H3.3 Antibody Phospho (pS31) - Protein Information | |
| Name H3F3A | |
| Synonyms H3.3A, H3F3 | |
| Function Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling | |
| Cellular Location Nucleus. Chromosome. | |
Histone H3.3 Antibody Phospho (pS31) - Related products
AM1151a: Phospho-Histone H3-S10 Antibody
AM1151b: Phospho-Histone H3-S10 Antibody
AM1151c: Phospho-Histone H3-S10 Antibody
AP1050a: Histone H3-MeK9 Antibody
AP1051d: Histone H3 Antibody (S10)
AP3667a: Phospho-H3-S10 Antibody
AP9934a: Histone H3-K9 Antibody
RI12389: H3F3A predesign siRNA
RI12390: H3F3B predesign siRNA
LY11083a: H3F3B Over-expression Lysate
BP1050a: Histone H3-MeK9 Antibody Blocking Peptide
BP1051a: Histone H3 (N-term) Blocking Peptide
BP1051d: Histone H3 Antibody (S10) Blocking Peptide
BP3667a: Phospho-H3-S10 Antibody Blocking Peptide
BP8890a: H3-S10 Antibody Blocking Peptide
BP9934a: Histone H3-K9 Antibody Blocking Peptide
Histone H3.3 Antibody Phospho (pS31) - Application data
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A. Western blot analysis on HeLa cell lysates using anti- Histone H3.3 Phospho (pS31) RabMAb (Cat. #AJ1354a). Cells were either (A) untreated (B) treated with Calyculin.
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B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Histone H3.3 RabMAb (Cat. #AJ1354a).
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C. Immunofluorescent staining of HeLa cells using anti-Histone H3.3 RabMAb (Cat. #AJ1354a).
Histone H3.3 Antibody Phospho (pS31) - Research Areas
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BACKGROUND
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
REFERENCES
1. Braunstein M, et al. Mol. Cell. Biol. 16:4349 2. Workman JL, et al. Annu. Rev. Biochem. 67: 545 3. Cheung, P. et al. Cell 103, 263 4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005 5. Albig W, et al. Hum Genet 97:486-491. 1996
