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Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | Q5QNW6 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone Names | EP857Y |
| Calculated MW | 13920 Da |
| Gene ID | 440689 |
| Other Names | Histone H2B type 2-F, HIST2H2BF |
| Target/Specificity | A synthetic acetylated peptide corresponding to residues surrounding Lys 5 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lys 5. |
| Dilution | WB~~1:50000~ IHC~~1:250~500 |
| Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Histone-H2B Antibody Acetyl (K5) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HIST2H2BF |
|---|---|
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Cellular Location | Nucleus. Chromosome. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).
References
1. Braunstein M, et al. Mol. Cell. Biol. 16:4349
2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545
3. Cheung, P. et al. Cell103, 263
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005.
5. Ahn SH, et al. Cell Cycle 4:780-783, 2005.
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