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Histone-H3.3 Antibody Phospho-pS28&31

Rabbit Monoclonal Antibody

  • WB - Histone-H3.3 Antibody Phospho-pS28&31 AJ1358a
    A. Western blot nalysis on HeLa cell lysates using anti-Phospho-Histone H3.3 (pS28 & S31) RabMAb (Cat. #AJ1358a), 1:5000 dilution. Cells were either (A) untreated (B) treated with Hela+FBS +calyculin A
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P84243
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1874Y
Calculated MW 15328 Da
Gene ID 3020;3021
Other Names Histone H33, H3F3A, H33A, H3F3
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding serine 28 and serine 31 of human Histone H3.3 protein.
Dilution WB~~1:2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHistone-H3.3 Antibody Phospho-pS28&31 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H3F3A
Synonyms H3.3A, H3F3
Function Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)

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Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes.


1. Braunstein M, et al. Mol. Cell. Biol. 16:4349
2. Workman JL, et al. Annu. Rev. Biochem. 67: 545
3. Cheung, P. et al. Cell 103, 263
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005
5. Albig W, et al. Hum Genet 97:486-491. 1996

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Cat# AJ1358a
(40 western blots)
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