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HMGB1 AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1364a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
HMGB1 Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | P09429 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EPR3507 |
| Calculated MW | 24894 Da |
| Gene ID 3146 | |
| Other Names HMGB1, HMG1, High mobility group protein B1, High mobility group protein 1 | |
| Target/Specificity A synthetic peptide corresponding to residues in human HMGB1 was used as an immunogen. | |
| Dilution WB~~1:10000~50000 IHC~~1:250~500 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions HMGB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
HMGB1 Antibody - Protein Information | |
| Name HMGB1 | |
| Synonyms HMG1 | |
| Function DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity) | |
| Cellular Location Nucleus. Chromosome. | |
HMGB1 Antibody - Related products
RI12510: HMGB1 predesign siRNA
AT2384a: HMGB1 Antibody (monoclonal) (M02)
HMGB1 Antibody - Application data
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A. Western blot analysis on (A) SKBR-3, (B) Hela and (C) HepG2 cell lysates using anti-HMGB1 RabMAb (Cat. #AJ1364a), dilution 1:50,000.
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B. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGB1 RabMAb (Cat. #AJ1364a).
HMGB1 Antibody - Research Areas
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BACKGROUND
High-mobility group box 1 protein (HMGB1) is a nuclear DNA-binding protein, which also functions as a pleiotropic cytokine, implicated in the pathology of several different immune-mediated diseases (1). As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription. Recent studies identified extracellular HMGB1 as a potent macrophage-activating factor, signaling via the receptor for advanced glycation end-products to induce inflammatory responses. HMGB1 mediates endotoxin lethality, acute lung injury, arthritis induction, activation of macrophages, smooth muscle cell chemotaxis, and epithelial cell barrier dysfunction (2).
REFERENCES
1. Kornblit B., et al. Tissue Antigens 70:151-156, 2007
2. Andersson U, et al. J Leukoc Biol. 72(6):1084-91, 2002
