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HMGB1 Antibody

Rabbit Monoclonal Antibody

  • WB - HMGB1 Antibody AJ1364b
    A.Western blot analysis on (A) SKBR-3, (B) HeLa and (C) Molt-4 cell lysates using anti-HMGB1 RabMAb (Cat. #AJ1364b), dilution 1:1000.
  • IHC - HMGB1 Antibody AJ1364b
    B. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HMGB1 RabMAb (Cat. #AJ1364b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09429
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3506
Calculated MW 24894 Da
Gene ID 3146
Other Names High mobility group protein B1, High mobility group protein 1, HMG-1, HMGB1, HMG1
Target/Specificity A synthetic peptide corresponding to residues in human HMGB1 was used as an immunogen.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHMGB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGB1
Synonyms HMG1
Function DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).
Cellular Location Nucleus. Chromosome.
Citations ( 0 )


High-mobility group box 1 protein (HMGB1) is a nuclear DNA-binding protein, which also functions as a pleiotropic cytokine, implicated in the pathology of several different immune-mediated diseases (1). As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription. Recent studies identified extracellular HMGB1 as a potent macrophage-activating factor, signaling via the receptor for advanced glycation end-products to induce inflammatory responses. HMGB1 mediates endotoxin lethality, acute lung injury, arthritis induction, activation of macrophages, smooth muscle cell chemotaxis, and epithelial cell barrier dysfunction (2).


1. Kornblit B., et al. Tissue Antigens 70:151-156, 2007
2. Andersson U, et al. J Leukoc Biol. 72(6):1084-91, 2002

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Cat# AJ1364b
(40 western blots)
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