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hnRNP C1/C2 Antibody

Rabbit Monoclonal Antibody

     
  • WB - hnRNP C1/C2 Antibody AJ1367a
    A.Western blot analysis on (A) HeLa, (B) 293T, (C) MCF-7 and (C) human cerebellum lysates using anti-hnRNP RabMAb (Cat. #AJ1367a), dilution 1:100,000.
  • IHC - hnRNP C1/C2 Antibody AJ1367a
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-hnRNP C1/C2 RabMAb (Cat. #AJ1367a).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P07910
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP3034Y
Calculated MW 33670 Da
Gene ID 3183
Other Names Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2, HNRNPC, HNRPC
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human hnRNP C1/C2 was used as an immunogen.
Dilution WB~~1:50000~100000
IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionshnRNP C1/C2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HNRNPC
Synonyms HNRPC
Function Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6- methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).
Cellular Location Nucleus. Note=Component of ribonucleosomes.
Citations (0)

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Background

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are predominantly nuclear RNA-binding proteins that form complexes with RNA polymerase II transcripts. These proteins function in a variety of cellular activities, ranging from transcription and pre-mRNA processing in the nucleus to cytoplasmic mRNA translation and turnover (1). hnRNP C1/C2 is one of the most abundant proteins in the nucleus, and shown to have roles in cellular differentiation and proliferation (2). A novel nuclear export is activated by the ROCK signaling pathway to exclude hnRNP C1/C2 from the nucleus, by which the compartmentalization of specific hnRNP components is disturbed in apoptotic cells (3). Overexpression of hnRNP C1/C2 in hepatocellular carcinoma (HCC) tissues has been reported in 70 HCC samples, suggesting its potential as a protein tumor marker (4).

References

1. Krecic AM, et al. Curr Opin Cell Biol. 11(3):363-71, 1999
2. Hossain M, et al. Molecular and Cellular Biochemistry 296(1-2):151-157, 2007
3. LEE Hsiao-Hui, et al. Journal of Cell Science 117(23):5579-5589, 2004
4. Wei Sun, et al. Molecular & Cellular Proteomics 6:1798-1808, 2007

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Discontinued
Cat# AJ1367a
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