|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||33670 Da|
|Other Names||Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2, HNRNPC, HNRPC|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human hnRNP C1/C2 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||hnRNP C1/C2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6- methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non- coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre- mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'- UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).|
|Cellular Location||Nucleus. Note=Component of ribonucleosomes.|
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Provided below are standard protocols that you may find useful for product applications.
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are predominantly nuclear RNA-binding proteins that form complexes with RNA polymerase II transcripts. These proteins function in a variety of cellular activities, ranging from transcription and pre-mRNA processing in the nucleus to cytoplasmic mRNA translation and turnover (1). hnRNP C1/C2 is one of the most abundant proteins in the nucleus, and shown to have roles in cellular differentiation and proliferation (2). A novel nuclear export is activated by the ROCK signaling pathway to exclude hnRNP C1/C2 from the nucleus, by which the compartmentalization of specific hnRNP components is disturbed in apoptotic cells (3). Overexpression of hnRNP C1/C2 in hepatocellular carcinoma (HCC) tissues has been reported in 70 HCC samples, suggesting its potential as a protein tumor marker (4).
1. Krecic AM, et al. Curr Opin Cell Biol. 11(3):363-71, 1999
2. Hossain M, et al. Molecular and Cellular Biochemistry 296(1-2):151-157, 2007
3. LEE Hsiao-Hui, et al. Journal of Cell Science 117(23):5579-5589, 2004
4. Wei Sun, et al. Molecular & Cellular Proteomics 6:1798-1808, 2007
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