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HSF1 Antibody Phospho (pS326)

Rabbit Monoclonal Antibody

  • WB - HSF1 Antibody Phospho (pS326) AJ1374d
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-HSF1 (pS326) RabMAb (cat. #2029-1). Cells were either (A) untreated (B) treated with heat (44°C)
  • IHC - HSF1 Antibody Phospho (pS326) AJ1374d
    C. Immunohistochemical analysis of paraffin-embedded human stomach using Phospho-specific anti-HSF1 RabMAb (Cat. #AJ1374d).
  • IHC - HSF1 Antibody Phospho (pS326) AJ1374d
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-HSF1 RabMAb.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q00613
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1713Y
Calculated MW 57260 Da
Gene ID 3297
Other Names Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1
Target/Specificity A phospho specific peptide corresponding to residues surrounding Ser326 of human HSF1 was used as an immunogen. This antibody detects HSF1 phosphorylated on Ser326.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSF1 Antibody Phospho (pS326) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSF1
Synonyms HSTF1
Function DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules
Research Areas
Citations (0)

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Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. (2). Phosphorylation of Ser326 but none of the other Ser residues was found to contribute significantly to activation of the factor by heat stress. Phosphorylation on Ser326 increased rapidly during heat stress as shown by experiments using a pSer326 phosphopeptide antibody. Mutagenesis experiments and functional studies suggested that phosphorylation of HSF1 residue Ser326 plays a critical role in the induction of the factor's transcriptional competence by heat stress. PhosphoSer326 also contributes to activation of HSF1 by chemical stress (3).


1. Rabindran Sk, et al. Proc Natl Acad 88(16):6906-10, 1991
2. Xing H, et al. J Biol Chem 279(11):10551-5, 2004
3. Guettouche T et al. BMC Biochem 11(6):4, 2005

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Cat# AJ1374d
(40 western blots)
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