|Calculated MW||60348 Da|
|Other Names||Heat shock factor protein 2, HSF 2, Heat shock transcription factor 2, HSTF 2, HSF2, HSTF2|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human HSF2 was used as an immunogen|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HSF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.|
|Cellular Location||Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation|
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Provided below are standard protocols that you may find useful for product applications.
The heat shock response is transcriptionally regulated by an evolutionarily conserved protein termed heat shock factor (HSF). It appears that there is a human heat shock factor gene family and that at least two separate but related HSF proteins regulate the stress response in humans (1). HSF2, one of the heat shock transcription factors in mammalian cells, is localized to the cytoplasm during normal growth and moves to the nucleus upon activation. Heat shock transcription factors in metazoans contain four hydrophobic heptad repeat sequences, three in the amino terminus and one in the carboxy terminus, which are predicted to form alpha-helical coiled-coil structures analogous to the leucine zipper. It is believed that zipper domains can regulate subcellular localization (2). The promoter regions of some genes, such as the stress-inducible hsp70i gene that codes for a heat shock protein, remain uncompacted, a phenomenon called bookmarking. It has been shown that hsp70i bookmarking is mediated by transcription factor HSF2 (3)
1. Schuetz TJ, et al Proc Natl Acad Sci 88(16):6911-6, 1991
2. Sheldon LA, et al. Genes Dev 7(8):1549-58, 1993
3. Xing H, et al. Science 307(5708):421-3, 2005
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