|Calculated MW||116598 Da|
|Other Names||Hormone-sensitive lipase, HSL, LIPE|
|Target/Specificity||A phospho-specific peptide corresponding to residues surrounding Serine 855 of human HSL was used as an immunogen. The antibody only detects HSL phosphorylated on Serine 855.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HSL Antibody Phospho (pS855) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.|
|Cellular Location||Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Note=Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation|
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Provided below are standard protocols that you may find useful for product applications.
Hormone-sensitive lipase (HSL) is a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis. It is acutely controlled through reversible phosphorylation by catecholamines and insulin (1). HSL is the rate-limiting enzyme in hydrolysis of triglycerides in adipose tissue and of cholesteryl esters in steroidogenic tissues including the adrenals, ovaries, testes, and macrophages (2, 3). HSL activity has been positively correlated with free and esterified cholesterol ratios in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions, but not with triglyceride levels, during testicular development. HSL is localized to elongated spermatids and Sertoli cells, where its distribution is stage-dependent, and within the cells lining the excurrent ducts of the testis. The HSL protein levels and enzymatic activity in ITf and STf were reported positively correlated with serum testosterone levels during development (4).
1. Holm C, et al. Science 241(4872):1503-6, 1988
2. Li Z, et al. Genomics 24(2):259-65, 1994
3. Osuga J, et al. Proc Natl Acad Sci U S A. 97(2):787-92, 2000
4. Kabbaj O, et al Biology of Reproduction 65:601-612, 2001
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