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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HSP27 Antibody   

HSP27 Antibody

Rabbit Monoclonal Antibody

     
  • WB - HSP27 Antibody AJ1378a
    A. Western blot analysis on HeLa cell lysate using anti-Hsp 27 RabMAb (Cat. #AJ1378a).
  • IHC - HSP27 Antibody AJ1378a
    C. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-HSP27 RabMAb. (Cat. #AJ1378a).
  • IHC - HSP27 Antibody AJ1378a
    B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-HSP27 RabMAb (Cat. #AJ1378a)
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P04792
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1724Y
Calculated MW 22783 Da
Gene ID 3315
Other Names Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human Hsp 27 was used as an immunogen.
Dilution WB~~1:1000~10000
IHC~~1:100
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSP27 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPB1
Synonyms HSP27, HSP28
Function Involved in stress resistance and actin organization.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
Tissue Location Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle
Research Areas
Citations (0)

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Background

Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).

References

1. Mendelsohn, M.E., et al. Proc Natl Acad Sci U S A. 88: 112-126 (1991).
2. Faucher,C.,et al..J.Biol Chem. 268:15168-73
3. Landry, J., et al. J. Biol. Chem. 267: 794-803 (1992)
4. Rouse, J., et al.. Cell 78: 1027-1037 (1994)
5. Latour, S et al. (1996) J. Biol. Chem. 271, 22782-90
6. Schymeinsky J, et al. PLos ONE 2(11), 2007

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Discontinued
Cat# AJ1378a
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